Structural and biochemical studies of influenza virus RNA-dependent RNA polymerase (FluPol) in complex with transcribing host RNA polymerase II reveal the molecular mechanisms of RNA cap snatching by FluPol.
Cloning and purification of proteins
To generate H7N9 FluPol with impaired endonuclease activity, the PA(E119D) mutation was introduced into the PA gene. A pFastBac Dual vector encoding the influenz… [+28523 chars]
